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KMID : 0381920100400020117
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Korean Journal of Microscopy
2010 Volume.40 No. 2 p.117 ~ p.123
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Experimental Examination of the Beer’s law for Quantitative Electron Tomography
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Kim Jin-Gyu
Kim Youn-Joong
Jou Hyeong-Tae
Song Kyung
Lee Su-Jeong
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Abstract
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This study has examined experimentally the Beer’s law which is a precondition for quantitative electron tomography. We used carbon support film and latex spheres, which have similar absorption coefficients with biological samples, as the test samples to take a tilt-series of images for electron tomography. First, the 3D information of carbon film and latex spheres was obtained by electron tomography. Then, the regression analysis on the relationship between the intensities of the incident and the transmitted beams in a tilt series was carried out to examine the Beer’s law. The regression results with RMS error of 0.976 show the linear intensity variations of the transmitted beam as the tilt angles were increased. In addition, the relative absorption coefficients of carbon support film and latex spheres calculated experimentally through the Beer’s law were 1.71 (5) and 2.67 (6)/μm, respectively. The absorption coefficients remained constant within a full tilt range. Therefore, it is expected that quantitative electron tomography could be performed for biological samples by applying Beer’s law provided the exact intensity of incident beam can be obtained under the thoroughly controlled experimental conditions.
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KEYWORD
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Beer’s law, Electron tomography, Latex sphere
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